RNF7 and pachyonychia congenita: The Transwell and wound-healing assay showed that overexpression of RNF7 enhanced the corresponding with that in the control group; in contrast, the opposite results exhibited in the group of PC cells treated with the PI3K/AKT signaling pathway inhibitor perifosine (5 μM) (p < 0.01) (Figures 5(c)–5(f)).