Additionally, we generated R83S CFH, which is a highly deleterious C3 glomerulopathy (C3G)-associated non-functional variant that was used a positive control for dysfunction (27) and H380R CFI, which was identified in a patient with clinical CFI deficiency (35) and was predicted to be inactive due to mutation of the FI active site (36). The gene discussed is CFH; the disease is complement 3 glomerulopathy.