According to the performance of the best Cut-off value, the diagnosis of AIS might be more inclined when S100A1 ≥181.03 pg/ml, while the diagnosis of TIA might be inclined when 150.14 pg/ml ≤S100A1 ≤181.03 pg/ml, and more inclined to normal when S100A1 ≤150.14 pg/ml. Here, S100A1 is linked to transient ischemic attack.