To validate enrichment pathways mentioned above, BCAT2 overexpression (BCAT2 OE) and BCAT2 knockdown (BCAT2 KD) human (T24)/murine (MB49) bladder cancer cell lines were constructed successfully (Figure S15A–D, Supporting Information) and were tested with high‐throughput RNA sequencing. The gene discussed is BCAT2; the disease is urinary bladder carcinoma.