Indeed, 3D cultivation conditions seem to improve neuronal development.[64, 134] We demonstrated that 3D cultivation techniques significantly accelerate 4R tau isoform expression in our 3D AD cellular models based on immortalized human neural progenitor cells.[9, 10] 3D ECM environments also accelerate 4R tau expression in human iPSC‐derived neuronal cultures, even though the adult brain 3R:4R tau ratio of 1:1 was not achieved.[135] These underscore advantage of using 3D culture models with ECM in modeling Alzheimer's tau pathology. This evidence concerns the gene MAPT and Alzheimer disease.