As predicted, stimulation of AgRP neurons (as shown by the increased c-Fos staining and food intake, Supplementary Fig. 2b–d) reversed CRS-induced anxiety-like behaviors with an increase in both center time duration and center distance in the OF test (Fig. 1a), and an increase in time and entries into the open arms in the EPM test (Fig. 1b). Here, AGRP is linked to Anxiety.