Our findings reveal, for the first time, that elevated SF3B4 promotes the export of KLF16 mRNA from the nucleus to the cytoplasm and thus enhances KLF16 expression, and in turn increased KLF16 binds to the Twist1 promoter to activate its transcription, leading to EMT and ccRCC cell migration. The gene discussed is TWIST1; the disease is nonpapillary renal cell carcinoma.