After 5 d of inoculation, either HDAC6 or GRP78 silencing showed a gradual reduction in the tumor spheroid growth rate, while double knockdown of both HDAC6 and GRP78 exhibited the greatest inhibitory effect (Fig. 7C, D), corresponding to an increase in tubulin acetylation and a decline in p-ERK expression (Fig. 7E, F). This evidence concerns the gene HSPA5 and neoplasm.