The GO enrichment analysis results suggest that NAFLD targets of metabolites from gut microbiota are mainly related to bile acid receptor activity, vitamin D 24-hydroxylase activity, the Sin3 complex, nucleosome remodeling and the deacetylase (NuRD) complex, the neutrophil apoptotic process, alkaloid catabolic process, dibenzo-p-dioxin metabolic process, and fungiform papilla formation to relieve NAFLD. This evidence concerns the gene CYP24A1 and metabolic dysfunction-associated steatotic liver disease.