To further explore whether the CUB1 domain of NETO2 can exert its inhibitory effect on tumor growth by changing the M2-like polarity transformation of TAMs, endogenic NETO2 was knocked out via CRISPR in RAW264.7-derived TAMs and His-NETO2-FL or His-NETO2-Del1 was transfected into targeted cells (Fig. 7a and Supplementary Fig. 4a). This evidence concerns the gene NETO2 and neoplasm.