Similarly, Western bloting (Fig. 5b and Supplementary Fig. 3c), ELISA (Fig. 5d and Supplementary Fig. 3d) and flow cytometry assays of cell lines (Fig. 5c and Supplementary Fig. 3e, f) and tumor single cell suspension (Fig. 5e) proved that, compared with the control group, the low expression of NETO2 in TAMs could block the effect of sLRIG3 and help maintain M2-like polarity transformation of TAMs, while the signature of M1-like markers decreased. This evidence concerns the gene NETO2 and neoplasm.