Deletion of either Eed or Ezh2 disrupted PRC2 function, as both M-Smo/EedcKO and M-Smo/Ezh2cKO showed minimal H3K27me3 in tumor cells, with residual H3K27me3 in interspersed stromal cells demonstrating the effectiveness of the staining technique (Fig. 5B). This evidence concerns the gene EED and neoplasm.