To evaluate flagella- and CBLB502-mediated NF-κB activation of 4T1 mammary carcinoma cells, we stably transfected 4T1 cells with a κB5:IκBɑ-FLuc transcriptional reporter comprised of a concatenated κB5 promoter region, followed by the bioluminescent IκBɑ-FLuc fusion reporter gene50,51. The gene discussed is NFKB1; the disease is breast carcinoma.