Based on partial least squares discriminant analysis (PLS-DA), we found that the proportion of two sorts of n-6 PUFAs, arachidonic acid (C20:4) and adrenic acid (C22:4), were significantly reduced in the circMBOAT2-silenced and FASN-silenced group (Fig. 7G), as a consequence of which, we explored the function of these two n-6 PUFAs in FASN-mediated ICC progression. Here, FASN is linked to intrahepatic cholangiocarcinoma.