Toobtain conformational information on fragile peptide aggregates, theinstrumental parameters of the TIMS-Quadrupole-Time-of-Flight massspectrometer (TIMS-qToF-MS) have to be optimized to allow the studyof intact aggregates and ensure their transmission toward the detector.Here, we investigate the suitability and application of TIMS to probethe aggregation process, targeting the well-characterized M307-N319peptide segment of the TDP-43 protein, which is involved in the developmentof amyotrophic lateral sclerosis. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.