The result as shown in Figure 3 reveals that the three genes arranged according to mutation frequency were APC Regulator of WNT Signaling Pathway (APC) (80.7%), Tumor Protein P53 (TP53) (63.5%), and Tenascin N (TTN) (63.5%) in CRC tissues (Figure 3a-3c). Here, TP53 is linked to colorectal carcinoma.