In comparison with the scrambled shRNA human ECs, the SATB2 shRNA knockdown human ECs showed a significant increase in senescence markers including both the SA‐β‐gal and the γ‐H2AX stainings (Figure 2d,e, left and middle panel), as well as the expression of the SASP genes IL‐6, IL‐1β, and ICAM‐1 (Figure 2f, left and middle lane) two days after shRNA lentiviral infection. This evidence concerns the gene H2AX and infection.