As shown in Figure 4, the expression of protein Rankl (Figures 4A,B) was significantly increased and the expressions of proteins BMP2 (Figures 4A,C), OPG (Figures 4A,D), OPN (Figures 4A,E), and Runx2 (Figures 4A,F) were significantly decreased in diabetic mice (Diabetes-control, D-control) compared with normal mice (Wild-control, W-control) (p < 0.05). Here, SPP1 is linked to diabetes mellitus.