After infection with the mRFP-GFP-LC3 adenovirus, the numbers of yellow and free red puncta in the si-IPMK+ebastine group were both significantly lower than those in the ebastine group, indicating that autophagosomes and autolysosomes had decreased in the three osteosarcoma cell lines (Fig. 6d-f; S9e). This evidence concerns the gene IPMK and infection.