Analysis by western blots, two-color flow cytometry, and real-time PCR detected that cisplatin and ischemic-induced AKI in NPY WT mice was associated with downregulation of Y1R while upregulating NF-κB/p65-Mincle signaling and Mincle+F4/80+ M1 macrophages, which became much more severe in mice lacking NPY (Figure 3e and Figure 5a, b-d). This evidence concerns the gene NFKB1 and acute kidney injury.