To determine if SRGN Exo promote invasion of ESCC cells, two ESCC cell lines with SRGN-overexpression (i.e. KYSE410-SRGN and KYSE150-SRGN) and their respective controls expressing empty vectors (i.e. KYSE410-Con and KYSE150-Con) were treated with DMA, an inhibitor of the H+/Na+ and Na+/Ca2+ exchangers, to inhibit exosome secretion. Here, SRGN is linked to esophageal squamous cell carcinoma.