The top upregulated proteins including teneurin-2 (TENM2), growth factor receptor bound protein 2 (GRB2), lectin mannose binding 1 (LMAN1), stromal cell derived factor 4 (SDF4), Golgi membrane protein 1 (GOLM1), M6PR and integrin α-5 (ITGA5), as well as two other proteins including EphB4 and neurogenic locus notch homolog protein 2 (Notch2) which were reported to be associated with cancer invasion 29, 30 were subjected to validation by Western blot. This evidence concerns the gene EPHB4 and cancer.