Conversely, activation of SIRT1 functionally interacts with AMPKα, up-regulates phosphorylation of AMPKα and then activates eNOS, inhibits ROS accumulation and oxidative stress in aortic ECs and leads to vascular remodeling, down-regulates collagen expression and elastin breakage, predicting the improvement of aortic sclerosis and hypertension 118. The gene discussed is NOS3; the disease is Hypertension.