NSUN2 and neoplasm: To further explore the tumor-promoting role of NSUN2 dependently on m5C modification in CC, we constructed a wide-type NSUN2 expression plasmid (NSUN2-WT) and an m5C methyltransferase-mutated plasmid (NSUN2-MUT) by introducing point mutations in releasing and catalytic sites (cysteine 271 and 321) (Fig. 6A).