GPD2 and neoplasm: As a result, the level of ether lipids, the final end metabolites by GPD2 reaction, was lower in the GPD2 KO tumor tissues (Figures 5E and S6), establishing the GPD2-ether lipid link in vivo. Furthermore, the level of phosphorylated Akt (p-Akt) and its downstream target, phosphorylated p70 S6K (p-p70 S6K), as assessed by Western blot, was also lower in the GPD2 KO tumor tissues (Figure 5F). Therefore, these data confirm GDP2-ether lipid-Akt axis in vivo.