FAM3C and adenocarcinoma: In contrast, most of adenocarcinoma or squamous cell lines showed high FAM3C content (Figure S4A), which is consistent with the scRNA-Seq database (Figure S3A). To delineate the role of FAM3C in NSCLC, we modulated its expression in the FAM3C-high SKMES-1 (using shRNA-mediated knockdown), FAM3C-low H2170 (using plasmid-based overexpression), and FAM3C-intermediate A549 cell lines (knockdown or overexpression).