Our transcriptome sequencing results also showed decreased Prox1 expression in LyECs from liver fibrosis mice, and the expression of the tight junction proteins ZO-1 (Tjp1) and Esam in LyECs was downregulated after liver fibrosis (Figure S6B), which indicated that the permeability of hepatic LyECs was affected. Here, ESAM is linked to Hepatic fibrosis.