Thus, evaluating SERCA1 alternative splicing in DM1 skeletal muscle cells is meaningful for considering the clinical situation, whereas recapitulation of SERCA1 mis-splicing in vitro seems to require a high level of maturity of the skeletal muscle cells and has been relatively difficult to do, even using primary cell cultures48,49. Here, ATP2A1 is linked to myotonic dystrophy type 1.