Although the results obtained by the molecular approach performed in the present study did not determine the variant allele, they should be interpreted with caution and need to be corroborated by independent and/or multicenter studies to determine the real prevalence of the -94ins/delATTG polymorphism in the NFKB1 gene and its association with NEC in the general population and also in the Brazilian population. Here, NFKB1 is linked to necrotizing enterocolitis.