The corresponding results of IHC showed that, compared with sham group, the UUO group damaged the renal tissue structure, promoted the dilatation of renal tubules and elevated the levels of renal fibrosis-related proteins LN (in tubules and glomeruli), FN (in tubules and interstitium), Col-I (in tubules and interstitium) and Col-III (in tubules and glomeruli) in the kidney tissue on days 14 and 21. Here, FN1 is linked to renal fibrosis.