Specifically, a positive HER2 result was IHC staining of 3+ (i.e., uniform, intense membrane staining of > 30% of invasive tumor cells), a fluorescent ISH (FISH) result > 6 HER2 gene copies per nucleus, or a HER2 gene to chromosome 17 signals ratio > 2.2; a negative result was an IHC staining of 0 or 1+, a FISH result of < 4.0 HER2 gene copies per nucleus, or FISH ratio < 1.8[7]. This evidence concerns the gene ERBB2 and neoplasm.