Fifteen genes had a |log2FC| > 2.0: ten genes (SIX2, PLEC1, ZNF833, RAI1, ZNF598, USP4, DOCK1, UNC84A, KLF16, PRKACA) were significantly hypermethylated, and five genes [STK33, TBXT (alias T), KCNH7, ADAMTS2, FAM19A5] were significantly hypomethylated in breast cancer tissues of cases compared to controls. The gene discussed is RAI1; the disease is breast cancer.