Our results indicated that the hallmark biomarkers of apoptosis (Bax/Bcl2, Cytochrome C and Cleaved-caspase 3/Caspase 3) (Fig. 5A–C), necroptosis (pRIPK3/tRIPK3, pMLKL/tMLKL) (Fig. 5D, E), and pyroptosis (NLRP3, GSDMD-NT/GSDMD) (Fig. 5H, I), but not ferroptosis (ACSL4) (Fig. 5G), were markedly increased in the myocardial tissue of the MI group in comparison with those of the sham group (Fig. 5). This evidence concerns the gene GSDMD and myocardial infarction.