GSDMD and hydrops fetalis: To support the evidence that a chronic ischemic insult could lead to myocardial death, contractile dysfunction, and inevitable HF, we determined the pertinent proteins of each type of programmed cell death pathway, including Bax, Bcl-2, Cytochrome C, Caspase 3, Cleaved-caspase 3, RIPK3, pRIPK3, MLKL, pMLKL, GPX4, ACSL4, NLRP3, GSDMD, and GSDMD-NT.