Alternatively, in a subgroup analysis of the NAFLD population, we found that the C allele of rs2279026 of the TBC1D1 gene, the G allele of rs2279028 of the TBC1D1 gene, the C allele of rs780093 of the GCKR gene, and the C allele of rs1260326 of the GCKR gene were strongly associated with increased risk of NAFLD in obesity. Here, GCKR is linked to obesity due to melanocortin 4 receptor deficiency.