Alternatively, in a subgroup analysis of the NAFLD population, we found that the C allele of rs2279026 of the TBC1D1 gene, the G allele of rs2279028 of the TBC1D1 gene, the C allele of rs780093 of the GCKR gene, and the C allele of rs1260326 of the GCKR gene were strongly associated with increased risk of NAFLD in obesity. Here, TBC1D1 is linked to metabolic dysfunction-associated steatotic liver disease.