To investigate the genetic basis of NAFLD versus non-NAFLD subjects, we found that genotypes and alleles at the rs1421085, rs8050136, rs3751812, rs9939609 for FTO, rs2206277 for TFAP2B, and rs1260326 for GCKR loci were associated with increased risk of NAFLD. Here, TFAP2B is linked to metabolic dysfunction-associated steatotic liver disease.