Thus, a recent study published by our group has established a relationship of reference genes in sheep suitable for infections with F. hepatica in the liver and HLN and, in addition, validates other genes related to proinflammatory mediators such as IL-1β and TNF-α, and regulators such as IL-10, TGF-β and FoxP3 [29]. The gene discussed is TGFB1; the disease is infection.