The aim of this study was to evaluate the quantification by qRT-PCR of the expression of pro-inflammatory (IL-1β, IFN-γ, TNF-α) and regulatory (IL-10, TGF-β, IL-4) cytokines, as well as the transcription factor and FoxP3, in the liver and hepatic lymph node (HLN) of sheep primoinfected and reinfected with F. hepatica during early and late stages of infection. This evidence concerns the gene TGFB1 and infection.