FLOT1 and colorectal cancer: While we were able to detect the presence of EV markers Syntenin-1 and Flotillin-1 in our positive control sample, concentrated media from colorectal cancer cells (DiFi), we were unable to detect these proteins in our DGUC-purified lipoproteins (i.e., VLDL, LDL, HDL) (Fig. 3A) (14).