MKI67 and squamous cell intraepithelial neoplasia: p16 and Ki-67 double immunostaining is used to detect high-grade squamous intraepithelial lesions (SIL).[18] The p16 and Ki-67 staining intensities gradually increase as lesions progress from low-grade to high-grade SIL.[19] Diffuse staining for p16 is a marker of high-grade SIL and is not associated with HPV infections.[20] p16 and Ki-67 positivity is associated with histologic severity.[21] Our case showed focal positivity for p16 and Ki-67, and no SIL was found.