Mice treated with intramuscular injection showed a greater reduction in tumour size (p<0.05 on days 18, 20, and 24) and (p<0.01 on days 26 and 28), which was concomitant with significantly higher infiltration of NY-ESO-1-specific CD8+ T cells in TIL (p<0.01) (Figure 5A), whereas mice infected via the intranasal route did not show any difference in tumour size (Figure 5A). Here, CD8A is linked to neoplasm.