Similarly, an increased frequency of Flu M1- or NY-ESO-1 specific IFNγ (single producer), or IFNγ and TNFα (double producer) or IFNγ, TNFα, and IL2-producing CD8+ T cells (triple producers) was observed in HHD mice spleen following intramuscular infection (Figure 3C), and conversely a higher frequency of flu M1- or NY-ESO-1-specific IFNγ or IFNγ and TNFα-producing CD8+ T cells was detected in HHD mice lungs following intranasal infection (Figure 3C). This evidence concerns the gene IFNG and infection.