EHD2 and breast cancer: By immunoblotting, we found EHD2 expression in immortal basal-like mammary epithelial cell lines 76Ntert (hTert-immortalized primary mammary epithelial cell line; Zhao et al., 2010) and MCF10A, in 2 out of 3 TNBC cell lines, and at lower levels in 3 out of 11 HER2 + cell lines, but in none of the 9 luminal A/B BC cell lines (Figure 2A).