However, in contrast to the NAFLD map, both pseudotime analyses showed more chaotic paths with different potential cellular endpoints, including the potentially cytotoxic Tr1 subcluster 3 (IL10, TIGIT, GZMH, GZMB, PRF1, GZMK) (Figure 2G and Supplemental Figure 2, C and D). The gene discussed is PRF1; the disease is metabolic dysfunction-associated steatotic liver disease.