To validate the observation regarding the increased frequency of liver CD4+ T cells able to coproduce multiple cytokines in NASH patients, we performed intracellular cytokine FACS staining on CD4+ T cells freshly isolated from the liver of 26 NAFLD and 39 NASH patients (Figure 4A, Supplemental Figure 5A, and Supplemental Table 3). This evidence concerns the gene CD4 and metabolic dysfunction-associated steatohepatitis.