To validate the observation regarding the increased frequency of liver CD4+ T cells able to coproduce multiple cytokines in NASH patients, we performed intracellular cytokine FACS staining on CD4+ T cells freshly isolated from the liver of 26 NAFLD and 39 NASH patients (Figure 4A, Supplemental Figure 5A, and Supplemental Table 3). The gene discussed is CD4; the disease is metabolic dysfunction-associated steatotic liver disease.