CD4 and metabolic dysfunction-associated steatohepatitis: By providing an unsupervised analysis, our study shows for the first time to our knowledge that the progressive inflammatory development behind the transition from NAFLD to NASH is not associated with only classical CD4+ Th cell subsets, as previously suggested (6, 30, 35, 36), but rather with a larger spectrum of different subsets, including multicytokine-producing cells that might represent in-between developmental stages.