To this end, we performed pharmacological and genetic inhibition of the exosomal key factor nSMase2 [55], and immunofluorescence microscopy analysis using Cy3-miR-141 or PKH67-labeled exosomes confirmed that the circulating miR-141 is derived from OvCa cells via the exosomal pathway, suggesting miR-141 possesses the capacity of mediating cell-to-cell communication in tumor-stroma interactions. This evidence concerns the gene SMPD3 and neoplasm.