VIM and hepatocellular carcinoma: We also extracted proteins from hepatocellular carcinomas, and adjacent normal tissue from CryabTg mice and normal liver tissue from control mice and performed targeted mass spectrometry to quantify EMT (Vimentin, α-Smooth muscle actin (α-SMA) and E-Cadherin) and angiogenesis (VEGFR, CD31, PDGFR) markers to validate whether the elevated level of Cryab expression is associated with high expression of these markers.