To determine the clinical relevance of IRE1α-XBP1 signaling in NSCLC patients, we developed a computational pipeline to specifically quantify the percentage of the spliced XBP1 mRNA isoform (XBP1s) relative to the total XBP1 transcript from RNA-seq data available in the TCGA database (Fig. 1a). The gene discussed is ERN1; the disease is non-small cell lung carcinoma.