Nevertheless, they have been used to measure responses to Wnt-11 in prostate and colorectal cancer cells (Murillo-Garzón et al., 2018; Gorroño-Etxebarria et al., 2019) and in chick embryo mesenchymal cells (Boitard et al., 2015), to Wnt-5a in osteosarcoma cells (Yamagata et al., 2012) and to collagen triple helix repeat containing-1, a protein that enhances Wnt interaction with FZD proteins and/or ROR2 (Zhang et al., 2017) in primary gastrointestinal stromal tumour (GIST) cells (Gorroño-Etxebarria et al., 2019). Here, CTHRC1 is linked to gastrointestinal stromal tumor.