Quantitative real-time polymerase chain reaction showed that the mRNA level of PAK4 was increased in CRC tissues and cells compared with para-cancerous tissues and CCD-841CoN cells, while decreased in the si-IGFL2-AS1-1 and si-IGFL2-AS1-2 groups compared with the si-NC group, elevated in the si-IGFL2-AS1-2 + inhibitor-433-3p group compared with the si-IGFL2-AS1-2 + inhibitor-NC, and increased in the oe-IGFL2-AS1 group compared with the oe-NC group (P  < .05, Figure 5C). This evidence concerns the gene PAK4 and colorectal carcinoma.