Stockhausen et al. stated that dedifferentiation (from differentiated cells - adhered to stem cells, neurospheres, in this case) reduces tumor proliferation and stabilizes neurospheres, signaling that EGFR blockade would increase the target on the GBM stem cells population, so it may suggest that TMZ intervention was effective on adhered cells, while stimulating the differentiation of NS in those submitted to IR [22]. The gene discussed is EGFR; the disease is glioblastoma.