To further explore the impact of PLK4 on immune cells infiltration, we evaluated the classical proportions of 9 types of infiltrating immune cells (M1 macrophages, B cells, Treg cells, CD8 T cells, CD4 T cells, NK cells, monocytes, dendritic cells, neutrophils) using the quanTIseq algorithm by Spearman’s rank correlation test in TCGA-GBM datasets. The gene discussed is CD8A; the disease is glioblastoma.