We detected the MRNA expression of cuproptosis-related signature in TCGA paired samples and found the expression of DLAT, PDHB, PDHA1 were significantly increased in cancer tissues when compared with cancer-adjacent tissues, while the expression of GLS and MTF1 trended downward, the decrease was not significant between paracancerous tissues and cancer tissues (Figure 4G). This evidence concerns the gene DLAT and cancer.