This allowed us to directly compare proximate areas of tumor with distinct immune cell biology (Figure 4B), and extract raw ion counts for each metabolite quantified in each ROI to obtain a direct comparison between those TME metabolic conditions favoring immune versus tumor cell proliferationWe compared 37 matched pairs of adjacent ROIs for CD3, 45 pairs for CD8, and 34 pairs for FOXP3, measuring relative metabolite abundance and pathway enrichment analysis with Metaboanalyst, a web-based interface for metabolomics data analysis (Xia et al., 2009; Chong et al., 2019; Pang et al., 2021). This evidence concerns the gene CD8A and neoplasm.