NOX1 and hereditary elliptocytosis: As shown in Figure 5A, 2-OH-E+ was clearly detected in the supernatant of NOX1-expressing cells, but was negligible in mock-transfected cells after a 60 min incubation with the HE probe at 37°C. Neither the level of E+, a non-specific oxidative metabolite of HE, nor that of HE itself was affected by the overexpression of NOX1 (Figure 5A).