By contrast, in the LightGBM algorithm, another gene, RNPEPL1 (cg16412000), is required to be hypomethylated, and the hypermethylation of this RNPEPL1 can be further used as a criterion for the decision rule of embryonal rhabdomyosarcoma. The gene discussed is RNPEPL1; the disease is embryonal rhabdomyosarcoma.