However, new sequence variants were observed near this region in the exon 3 of LEP. Analysis of rs28954114 (+328G/A) in the 3′-UTR of LEP revealed that the mutant allele A was observed in one subject with obesity (P076) and two control subjects (C145 and C248) in heterozygous condition (Figures 3(a)–3(f)). This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.